Mangone Lab

• One of the fundamental questions in molecular biology is how genes and the modulation of their expression are able to produce life in such incredible variety. • The mRNA molecule is the key intermediate of this process, as it is a perfect copy of the information contained in DNA, and instructs its temporal and spatial expression – producing proteins, shaping organs, timing development, and regulating biological processes in living organisms • RNA transcription has been studied for more than half a century, focusing mainly on the initiation and elongation processes. We still do not understand, however, the mechanisms of transcriptional termination, preparation of 3' mRNA ends, or the regulation of these sequences that in turn control gene expression at a post-transcriptional level. • The long-term goal of our laboratory is to highlight these fundamental biological mechanisms using a systems biology approach, taking advantage of biochemistry, high-throughput genomics and the powerful genetics provided by the round worm C. elegans and human cell lines.